RR-10 Mission: Mouse Kidney Transcriptomic and Proteomic Profiles Under Microgravity

Twenty mice flown on the Rodent Research-10 mission for 28-29 days, with matched ground control groups, provide kidney tissue data. The dataset includes transcriptional profiling from three sequencing methods and protein/phosphoprotein expression profiling using iTRAQ. It was generated by the National Aeronautics and Space Administration, with metadata last updated in March 2026.

Use Cases

• Identify gene expression changes in kidney tissue based on transcriptional profiling data from three sequencing methods.
• Compare protein and phosphoprotein abundance between flight and ground control groups using iTRAQ quantification.
• Analyze the effect of the p21-null genotype on molecular responses to microgravity across multiple data layers.
• Study time-course molecular markers of bone regeneration via pre- and in-flight injections of Alizarin Red and Calcein.

Strengths

• Includes data from 20 flight mice and three control groups (Basal, Vivarium, Ground) with 10-12 mice per group per strain.
• Provides multi-omics data from the same tissue samples, split for RNA and protein analysis.
• Experimental design includes precise timing of marker injections (e.g., Calcein at 7 days and 48 hours pre-euthanasia).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Protein expression and phosphoprotein profiling datasets exclude the Vivarium control group.

Provenance

Source

National Aeronautics and Space Administration

Collection Method

Data generated from kidney tissues of B6129SF2/J Wild Type and p21-null mice flown on the SpaceX-21 mission to the ISS, with matched ground controls. Tissues were pulverized and analyzed via 3' tag-Seq, polyA-enriched RNA-Seq, ribodepleted RNA-Seq, and iTRAQ proteomics.

Time Range

The RR-10 mission involved a 28-29 day microgravity exposure.

Freshness

Last updated 2026-03-13 19:45:27.886928; freshness should be verified.

Geography

Samples originated from mice on the International Space Station and in ground-based simulators.