Transcriptome of Porcine Muscle Stem Cells in a Duchenne Muscular Dystrophy Model

RNA sequencing data from myogenic skeletal muscle stem cells (mSCs) isolated from wild-type, DMD, and heterozygous pigs at fetal and 3-day postnatal stages. The dataset, created by Sophie Franzmeier and deposited in 2026, includes samples from intercostalis and pectoralis muscles cultivated in vitro to isolate cell-intrinsic changes. Sequencing was performed on the Illumina NextSeq550Dx platform and is available via GEO (GSE287756) and ArrayExpress (E-MTAB-16179).

Use Cases

• Identify differentially expressed genes in DMD versus wild-type muscle stem cells based on genotype comparisons mentioned in the description
• Analyze developmental stage-specific gene expression patterns based on the fetal and postnatal time points
• Compare transcriptomic profiles between intercostalis and pectoralis muscle-derived stem cells based on the two muscle biopsy sources

Strengths

• Data is isolated from the dystrophic environment via in vitro cultivation, allowing detection of cell-intrinsic changes.
• Includes samples from two distinct developmental stages (fetal and 3 days postnatal) and three genotypes (WT, DMD, HET).
• Deposited in two major public repositories (GEO and ArrayExpress) with specific accession numbers (GSE287756, E-MTAB-16179).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The 5.9 MB size suggests a relatively small dataset, potentially limiting statistical power for some analyses.

Provenance

Source

Sophie Franzmeier

Collection Method

RNA extracted from in vitro cultivated muscle stem cells and sequenced on an Illumina NextSeq550Dx platform.

Freshness

Last updated 2026-05-21 09:54:54; freshness should be verified.