Mouse Heart Transcriptomics After 30 Days on the International Space Station

RNA sequencing data from the hearts of female C57BL/6J mice flown on the International Space Station for 30 days. The dataset, produced by NASA, includes results from three flight mice and three ground control mice. Analyses identified 1147 significantly regulated transcripts and predicted activation of specific signaling pathways.

Use Cases

• Identify gene expression changes in cardiovascular tissue based on the 1147 significantly regulated transcripts.
• Analyze pathway activation in response to microgravity based on the described MAPK, PI3K-Akt, and GPCR signaling pathways.
• Study adaptive cellular responses to long-term spaceflight based on transcripts related to cytoskeleton, cell cycle, and cellular maintenance.
• Compare spaceflight effects on specific biological processes based on the reported absence of change in ECM components and oxidative stress pathways.

Strengths

• Data is derived from a controlled experiment with three flight and three ground control mice, enabling direct comparison.
• Analysis provides specific quantitative results, including 1147 significantly regulated transcripts.
• Dataset originates from NASA, an authoritative source for spaceflight research.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

National Aeronautics and Space Administration

Collection Method

RNA sequencing performed on heart tissue from mice flown on the ISS and ground controls.

Time Range

Experimental period involved a 30-day spaceflight.

Freshness

Last updated 2026-03-13 20:02:31.487697; freshness should be verified

Geography

Data originates from an experiment conducted on the International Space Station.