TRPC6-Mediated Calcium Signaling and Inflammatory Responses in Human Disc Cells

69.0 KB of raw and processed experimental data supports a study on TRPC6-mediated calcium influx in human intervertebral disc cells. Data includes calcium flux kinetics, cell viability, gene and protein expression, and pathway phosphorylation from primary human cells treated with the agonist Hyp9. The dataset was authored by Janitri Venkatachala Babu and last updated in April 2026.

Use Cases

• Analyze calcium influx dynamics based on real-time ΔF/F₀ and AUC measurements.
• Model inflammatory and catabolic gene expression based on qPCR data for markers.
• Investigate MAPK and NF-κB pathway activation based on Western blot densitometry.
• Assess cell viability and cytotoxicity effects based on PrestoBlue and LDH assay data.

Strengths

• Data is structured into seven distinct experimental modules covering multiple assay types.
• All data is associated with a specific manuscript, providing clear experimental context.
• File size is 69.0 KB, indicating a focused and manageable dataset for analysis.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Data is derived from primary human cells from an unspecified number of donors, which may limit generalizability.

Provenance

Source

figshare

Collection Method

Generated using primary human intervertebral disc cells following pharmacological activation of TRPC6 with Hyp9.

Time Range

Experimental timeframe not specified.

Freshness

Last updated 2026-04-13 12:45:34; freshness should be verified.

Geography

Spatial coverage not specified.