Trypanosoma brucei Bloodstream Form Protein Localisation Data with Microscopy Index

Endogenous mNeonGreen tagging data for protein subcellular localisation in the bloodstream form of the eukaryotic pathogen Trypanosoma brucei. The master deposition includes summary tables of localisations, primer sequences, and DOI indexing for microscopy data spread across multiple Zenodo repositories. Clare Halliday from the University of Oxford led this targeted tagging project, analogous to the genome-wide TrypTag procyclic form study.

Use Cases

• Predicting protein function based on subcellular localisation annotations provided in the localisations.tsv table.
• Linking gene IDs to raw microscopy data using the DOI index tables for image-based analysis.
• Analyzing gene selection criteria for tagging experiments using the geneselection.tsv table.
• Replicating the database structure for targeted tagging projects using the provided internal code in the master zip file.

Strengths

• Provides a structured index linking gene IDs to specific Zenodo DOIs for microscopy data.
• Includes primer sequences and tagging plate organization details for experimental reproducibility.
• Follows a directory structure analogous to a prior genome-wide project, aiding cross-study comparison.

Limitations

• The master deposition does not contain the microscopy images themselves; they must be downloaded separately from indexed DOIs.
• Column-level documentation is absent; field semantics must be inferred from file descriptions.
• Row count and total data size are unknown, which may limit suitability assessment.

Provenance

Source

University of Oxford, via the paperswithcode platform and Zenodo repositories.

Collection Method

Data generated from widefield epifluorescence microscopy of endogenously tagged proteins in Trypanosoma brucei bloodstream forms.