Uptake of SmartFlare Nanoparticles in HeLa Cells: Transmission Electron Microscopy Images

Transmission Electron Microscopy images from an open science project investigating the fate of Cy3-VEGF SmartFlare nanoparticles in HeLa cells. The dataset documents cells incubated with the nanoparticles for 18 hours, fixed, and processed with osmium and uranyl acetate staining. Images were captured on a Tecnai G3 Spirit microscope at both low (~2900x) and higher (~6800x) magnification.

Use Cases

• Analyze nanoparticle localization within cells based on TEM images described in the protocol.
• Train computer vision models to identify cellular structures and nanoparticle aggregates based on the staining and imaging methods.
• Study the reliability of SmartFlare probes for intracellular mRNA detection based on the experimental results.
• Compare cellular morphology and nanoparticle distribution between different magnifications mentioned in the description.

Strengths

• Detailed experimental protocol is provided, including cell seeding density (500,000 cells per well) and incubation time (18 hours).
• Imaging was performed on a specific microscope model (Tecnai G3 Spirit) at defined magnifications (~2900x and ~6800x).
• Sample preparation involved specific fixation and staining steps (e.g., 1% paraformaldehyde, 3% glutaraldehyde, osmium staining).

Limitations

• Row count and dataset size are unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Last update date is unknown; freshness unverified.

Provenance

Source

paperswithcode

Collection Method

Experimental data from cell culture, labeling, fixation, and TEM imaging as part of an open science project.