USP34/eIF3m/MTCH2 Axis: Microscopy Images of Mitochondrial Function in Breast Cancer Cells

129.2 MB of TIF microscopy images from a study on the role of ubiquitin-specific protease 34 (USP34) in triple-negative breast cancer. The research, authored by Peng-Fei Qian and last updated in April 2026, used assays like JC-1 and MitoSOX Red to show USP34 silencing inhibits cell proliferation by inducing mitochondrial dysfunction. Images likely capture mitochondrial membrane potential, superoxide levels, and network structure in TNBC cells.

Use Cases

• Training image segmentation models to identify mitochondrial structures based on Mito-Tracker Red CMXRos staining.
• Analyzing correlations between mitochondrial morphology and cell proliferation states based on the 5-ethynyl-2’-deoxyuridine assay results.
• Validating computational predictions of protein-protein interactions (e.g., USP34, eIF3m, MTCH2) with experimental co-immunoprecipitation and pull-down assay imagery.

Strengths

• Images are associated with a specific, peer-reviewed research hypothesis on the USP34/eIF3m/MTCH2 axis.
• Data is shared under a permissive CC-BY-4.0 license, allowing for reuse and redistribution.
• The 129.2 MB file size suggests a medium-scale collection of high-resolution microscopy images.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for machine learning tasks.
• The dataset's focus is on a specific molecular pathway in a single cancer type, limiting generalizability.

Provenance

Source

Peng-Fei Qian via figshare

Collection Method

Generated from laboratory assays including JC-1, MitoSOX Red, Mito-Tracker Red CMXRos staining, co-immunoprecipitation, and RNA-pull down analysis.

Time Range

null

Freshness

Last updated 2026-04-23 13:02:12; freshness should be verified.

Geography

null