Mouse Immunoglobulin Gene Repertoire Data from the International Space Station

Immunoglobulin gene sequencing data from C57Bl/6J mice, generated to validate sample preparation and bioinformatic methods for spaceflight research. The dataset was produced by the National Aeronautics and Space Administration using Illumina MiSeq and HiSeq platforms. It includes information on IgH and Igκ variable gene usage, junctional regions, and gene-segment modifications.

Use Cases

• Validate bioinformatic workflows for immunoglobulin repertoire analysis based on the described optimized pipeline.
• Compare immunoglobulin gene-segment usage and diversity between different sample preparation methods as described in the study.
• Assess the impact of sample storage constraints on sequencing outcomes for spaceflight experiments.
• Analyze junctional regions and modifications contributing to antibody specificity using the described NGS data.

Strengths

• Dataset is associated with a NASA-led study on spaceflight immunology.
• Bioinformatic workflow is specifically optimized for Illumina HiSeq and MiSeq datasets to reduce bias.
• Focuses on validating methods for a constrained environment (International Space Station).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data is stored in a BIN file format, which may require specialized tools to access.

Provenance

Source

National Aeronautics and Space Administration

Collection Method

Illumina MiSeq and HiSeq sequencing of tissue samples from C57Bl/6J mice, followed by a specific bioinformatic workflow.

Freshness

Last updated 2026-03-13 19:27:01.187684; freshness should be verified.