Yeast Display Macrocyclic Peptide Inhibitors of Human ACE2

2026 data from Zhanna Romanyuk details the discovery of low-nanomolar macrocyclic peptide inhibitors of human angiotensin-converting enzyme 2 (hACE2). The dataset includes results from screening millions of disulfide-cyclized peptide ligands using yeast display technology, identifying inhibitors with Ki values of 1.9 and 1.5 nM. It supports structural analysis of peptide binding modes distinct from previously reported inhibitors.

Use Cases

• Analyze Ki values (1.9 nM, 1.5 nM) to compare inhibitory potency of one-ring and two-ring macrocyclic peptides.
• Study the structural motifs (β-hairpin, cysteine-stabilized α-helix/α-helix) adopted by the two MPs in complex with hACE2.
• Use yeast display screening results to validate the technology for generating macrocyclic peptides with desired binding properties.
• Compare binding modes of the discovered MPs to those of previously reported hACE2 inhibitors.

Strengths

• Identifies macrocyclic peptide inhibitors with potent Ki values of 1.9 and 1.5 nM.
• Results from screening millions of structurally diverse disulfide-cyclized peptide ligands.
• Includes crystal structure data revealing distinct binding motifs for the discovered peptides.

Limitations

• Dataset size is very small at 2.2 KB, indicating limited scope and likely summary-level data.
• No column or sample data is provided, limiting direct analytical utility.
• Data is derived from a specific experimental study, not a broad survey or time-series.

Provenance

Source

Zhanna Romanyuk via figshare.

Collection Method

Quantitative screening of peptide libraries using yeast display technology.

Freshness

Last updated 2026-03-24.