MoClo YSD Toolkit 2.0: Parts for Yeast Secretion and Surface Display

An updated modular cloning toolkit for Saccharomyces cerevisiae, described in a 2026 publication. The dataset, shared by author Vanja Jurić, includes new genetic parts for optimizing protein secretion and surface display. It is a small dataset (53.2 KB) stored in an XLSX file.

Use Cases

• Optimizing heterologous protein secretion in yeast based on the described signal peptide and secretion-boosting protein parts.
• Designing yeast surface display systems for protein-protein interaction analysis using the described anchor protein and epitope tag options.
• Engineering whole-cell biocatalysts for plastic degradation based on the showcased co-display of PET-degrading enzymes.
• High-throughput screening of secreted proteins using the described GFP fluorescence complementation parts.

Strengths

• Dataset is associated with a peer-reviewed scientific publication describing the toolkit's applications.
• The toolkit version (2.0) and specific improvements, such as new anchor protein fusions and epitope tags, are clearly stated.
• File format is a standard, machine-readable XLSX spreadsheet.

Limitations

• Row count and column-level documentation are unknown, which may limit suitability assessment.
• Description metadata is limited; actual data quality and structure require manual inspection after download.

Provenance

Source

figshare

Collection Method

Likely contains the genetic part sequences, identifiers, and experimental design data supporting the described toolkit.

Freshness

Last updated 2026-04-23 23:46:06; freshness should be verified.