MoClo YSD Toolkit 2.0: Yeast Secretion and Surface Display Data

A modular cloning toolkit for Saccharomyces cerevisiae facilitates secretion and surface display of heterologous proteins. The dataset, authored by Vanja Jurić and updated in April 2026, includes new parts for anchor proteins, epitope tags, and secretion-boosting proteins. It demonstrates applications for analyzing protein-protein interactions and whole-cell biocatalysis of plastic degradation.

Use Cases

• Optimizing signal peptides and anchor proteins for protein secretion based on the modular cloning toolkit.
• Evaluating protein-protein interactions via yeast surface display of an anti-GFP nanobody mentioned in the description.
• Developing whole-cell biocatalysts for plastic degradation, as shown with PET-degrading enzyme codisplay.
• High-throughput detection of secreted proteins using GFP fluorescence complementation parts described.

Strengths

• Includes new parts for anchor proteins with three epitope tag options, as described.
• Demonstrates concrete applications for protein interaction analysis and plastic degradation biocatalysis.
• Released under a CC-BY-NC-4.0 license, permitting non-commercial reuse with attribution.

Limitations

• Row count and column-level documentation are unknown, limiting suitability assessment.
• The dataset is small at 2.1 MB, which may indicate limited scale.
• Description metadata is limited; actual data structure and quality require manual inspection after download.

Provenance

Source

figshare

Collection Method

Likely contains experimental data and plasmid constructs from a synthetic biology research project.

Freshness

Last updated 2026-04-23 23:46:07