CCALNN-PEG Influence on Gold Nanoparticle Uptake in HeLa Cells

HeLa cells were incubated with 5 nm gold nanoparticles coated with varying proportions of CALNN and CCALNN-PEG peptides. The dataset consists of two-plane TIFF images showing nanoparticle uptake, with one plane for brightfield and another for photothermal imaging. The data was generated by Yann Cesbron and is associated with a scientific paper on the paperswithcode platform.

Use Cases

• Quantifying nanoparticle uptake efficiency based on peptide coating ratios described in the image filenames.
• Training image segmentation models to identify nanoparticles in photothermal microscopy images.
• Analyzing the correlation between brightfield cell morphology and photothermal signal from nanoparticles.
• Comparing cellular uptake patterns across different surface ligand mixtures (e.g., 0% vs. 30% CCALNN-PEG).

Strengths

• Images are paired brightfield and photothermal planes, allowing for direct visual correlation.
• Experimental conditions are precisely described, including nanoparticle size (5 nm), concentration (100 nM), and incubation times.
• Data covers four distinct peptide coating mixture ratios (0%, 10%, 20%, 30% CCALNN-PEG), enabling comparative analysis.

Limitations

• The total number of images (rows) is unknown, which may limit suitability assessment for large-scale model training.
• Column-level documentation is absent; image metadata and processing details must be inferred after download.
• Data may reflect experimental bias inherent to the specific cell line (HeLa) and conditions used in the associated paper.

Provenance

Source

Yann Cesbron (author), associated with a paper on paperswithcode.

Collection Method

HeLa cells incubated with peptide-coated gold nanoparticles and imaged via photothermal microscopy.