KDO1: Crystal Structure of an Fe(II)/Alpha-Ketoglutarate Dependent Dioxygenase

T. Isabet from the Centre National de la Recherche Scientifique reports the crystal structure of the enzyme KDO1, a member of the Clavaminate Synthase Like family. The data includes structural elucidation of KDO1 and KDO5 in complex with their ligands, revealing active site features controlling regioselectivity. This study provides a basis for exploring the functional diversity of this enzyme family.

Use Cases

• Predicting enzyme regioselectivity based on active site structural features mentioned in the description
• Modeling enzyme-substrate interactions for the CSL family based on the described lid and sub-pocket dynamics
• Classifying active sites within the αKAO enzyme family using the structural and computational analysis framework described

Strengths

• Structure includes enzyme complexes with Fe(II) and alpha-ketoglutarate ligands
• Analysis covers two new regiodivergent enzyme members, KDO1 and KDO5

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download

Provenance

Source

Centre National de la Recherche Scientifique

Collection Method

Structural elucidation via X-ray crystallography and computational analysis