Crystal Structure of Yeast Nrd1 CID in Complex with Sen1 NIM3

The dataset contains structural biology data from a study by Yinglu Zhang at Columbia University. It details the crystal structures of the Nrd1 CID domain bound to Sen1 NIM motifs, characterizing interactions in vitro and in yeast. The data underpins findings on the NNS complex's role in non-coding RNA transcription termination.

Use Cases

• Modeling protein-protein interactions based on the described crystal structures.
• Studying transcription termination mechanisms based on the NNS complex interactions.
• Validating in vitro binding assays with structural data from the described motifs.

Strengths

• Data is derived from crystallography, providing high-resolution structural details.
• Interactions characterized both in vitro and in a yeast model system.
• Study identifies three specific sequence motifs (NIMs) mediating the interaction.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Last update date is unknown; freshness unverified.

Provenance

Source

Columbia University

Collection Method

Crystallography and in vitro/in vivo characterization.