Development of Rapid Multiplex Human Herpesvirus Detection Systems

A research document describes three molecular detection systems for human herpesviruses (HHVs) developed by Yidan Sun. The systems include an Octaplex PCR, a Quadruplex RPA, and a Duplex RPA-LFA assay, each with defined sensitivity levels and reaction times. The document was last updated on 2026-05-26.

Use Cases

• High-throughput contamination screening of cell samples based on the described Octaplex PCR system
• Rapid preliminary screening for HHVs based on the Quadruplex RPA system's 15-20 minute reaction time
• On-site visual detection of viruses without complex instruments based on the Duplex RPA-LFA system

Strengths

• The Octaplex PCR system has a defined sensitivity of 1 × 10^4 copies per 50 μL system
• The Quadruplex RPA system improved sensitivity to 1 × 10^3 copies per 50 μL system, which is 10 times higher than the PCR system
• The Duplex RPA-LFA system enables visual result detection within 20 minutes

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• The dataset is a 189.0 KB DOCX file, indicating a very limited scope of raw data
• Row count is unknown, which may limit suitability assessment

Provenance

Source

Yidan Sun

Collection Method

The detection systems were constructed by combining multiplex PCR with Recombinase Polymerase Amplification (RPA) and Lateral Flow Assay (LFA).

Freshness

Last updated 2026-05-26 06:02:09; freshness should be verified