Klebsiella Pneumoniae K27 Capsule Degradation and Biofilm Data

Magdalena Pelka's 2026 research data characterizes a novel phage-derived depolymerase, PRA33gp45, targeting Klebsiella pneumoniae. The 113.4 MB dataset contains experimental results on capsule hydrolysis, biofilm inhibition, and virulence reduction in Galleria mellonella models. It includes TIF microscopy images, XLSX analysis data, and DOCX documentation.

Use Cases

• Analyze confocal microscopy TIF images to quantify changes in biofilm architecture after depolymerase treatment.
• Model the relationship between depolymerase treatment time (e.g., 120-minute capsule degradation) and bacterial survival rates in A549 cell assays.
• Correlate halo zone measurements from bacterial lawn assays with capsular polysaccharide hydrolysis efficacy for the K27 serotype.
• Assess the impact of serum complement-mediated killing data on predicting mortality outcomes in Galleria mellonella infection models.

Strengths

• Data includes multiple experimental modalities: quantitative assays, confocal microscopy images, and larval model outcomes.
• Specific focus on the K27 serotype of Klebsiella pneumoniae, a clinically relevant target.
• Dataset is openly licensed under CC-BY-4.0 for broad reuse.

Limitations

• Sample sizes for experimental replicates are not specified in the provided metadata.
• Data is confined to a single bacterial serotype (K27), limiting generalizability.
• The temporal coverage of the experiments is not defined beyond the publication date.

Provenance

Source

figshare, authored by Magdalena Pelka.

Collection Method

Experimental laboratory research involving bioinformatic analysis, recombinant protein production, bacterial assays, microscopy, and in vivo models.

Time Range

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Freshness

Last updated in April 2026.

Geography

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