Fluorophore-Doped Polymer Substrates for Screening Plastic-Degrading Enzymes

14 PET hydrolases were evaluated using a high-throughput fluorescence assay to screen for plastic-degrading activity. The dataset, published by Anton A. Stepnov on figshare in 2026, contains results from testing rhodamine 6G-doped PET, polyethylene, and nylon substrates. Fluorescence data from enzymatic depolymerization correlated with quantitative chromatographic analysis of degradation products.

Use Cases

• Benchmarking new plastic-degrading enzymes based on fluorescence release data from doped substrates.
• Developing high-throughput screening protocols for enzyme activity in crude cell lysates based on the described method.
• Evaluating substrate suitability for different polymer types (PET, polyethylene, nylon) based on fluorophore leaching results.

Strengths

• Method validated against quantitative chromatographic analysis for 14 PET hydrolases.
• Demonstrated low background signals for detecting PETase activity in crude cell lysates.
• Dataset size is 13.4 MB, facilitating quick download and inspection.

Limitations

• Row count and column-level documentation are absent; field semantics must be inferred after download.
• The method showed limited utility for detecting nylonase activity due to rapid fluorophore leaching.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Experimental data from enzymatic hydrolysis assays of fluorescent dye-doped polymers.

Freshness

Last updated 2026-03-25 19:08:52; freshness should be verified.