Rice TMS5 Mutant RNA-seq: Thermosensitive Fertility

RNA-seq data from a study on TMS5-mediated R-loop dynamics in rice. The dataset profiles transcriptomes from Oryza sativa ssp. japonica wild-type Zhonghua11 and a CRISPR/Cas9-generated tms5 mutant under different temperature conditions, with three biological replicates per condition. It was used to identify differentially expressed genes and biological processes related to temperature-dependent anther development and fertility conversion.

Use Cases

• Identifying differentially expressed genes between wild-type and tms5 mutant rice under varying temperatures.
• Investigating transcriptional regulatory programs associated with tapetum function in anther development.
• Validating RNA-seq findings through downstream RT-qPCR or cytological analyses.
• Studying the role of R-loop dynamics in plant fertility and stress responses.

Strengths

• Includes three biological replicates per condition, supporting statistical robustness.
• Data is associated with a specific, peer-reviewed study on a defined molecular mechanism (TMS5/R-loop).
• Available under a permissive CC-BY-4.0 license for reuse.

Limitations

• Column names and exact row counts are not provided in the available metadata.
• The dataset is large (approx. 21 GB in GZ format) which may require significant storage and computational resources.
• Metadata is sparse, lacking details on specific temperature conditions, developmental stages sampled, or data processing pipelines.

Provenance

Source

Ben-Shun Zhu

Collection Method

RNA sequencing of total RNA extracted from rice anther tissues.

Freshness

Last updated on 2026-03-20.