Single-Cell RNA-seq of Colorectal Cancer-Macrophage Hybrid Cells and Parental Controls

Two biological replicates of in vitro fusion-derived mouse hybrid cells and their parental controls, profiled by 10x Genomics single-cell RNA-seq. The data was generated by Melissa Wong and submitted to Harvard Dataverse, with a last update recorded on 2026-04-14. The study compares hybrid cells with parental tumor and immune controls and includes artificial doublets for technical distinction.

Use Cases

• Identify transcriptomic signatures of tumor-macrophage hybrid cells based on single-cell RNA-seq data.
• Compare gene expression profiles between hybrid cells and parental tumor or immune controls.
• Distinguish bona fide hybrid cells from technical artifacts using artificial doublet data mentioned in the description.
• Analyze the effects of serial passaging on hybrid cell populations (hybrid-M vs. hybrid-T).

Strengths

• Includes two independent biological replicates for experimental validation.
• Contains multiple cell populations: two hybrid types (hybrid-M and hybrid-T) and both parental controls.
• Data was generated using the 10x Genomics Single Cell 3' Gene Expression kit and sequenced on an Illumina NextSeq 2000.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Data is derived from a mouse model (MC38 cells), which may limit direct translation to human biology.

Provenance

Source

Harvard Dataverse

Collection Method

Single-cell RNA-seq of FACS-isolated cells from co-culture experiments.

Time Range

Experimental timeframe not specified; dataset last updated in 2026.

Freshness

Last updated 2026-04-14 20:36:25; freshness should be verified.

Geography

Laboratory-based in vitro study; geographic origin not specified.