Salmonella Detection Data: RPA-Based Chromogenic Assay Results

A 1-hour detection method for Salmonella with a limit of detection as low as 12 CFU/ml. The dataset, authored by Chang Liu and last updated in April 2026, describes a label-free, equipment-free assay integrating recombinase polymerase amplification with DNAzyme catalysis for visual color change. Results include specificity tests against non-target bacteria like E. coli and Listeria.

Use Cases

• Benchmarking rapid pathogen detection methods based on the described RPA and G-quadruplex DNAzyme assay.
• Developing field-deployable biosensors for foodborne illness monitoring based on the equipment-free, visual detection platform.
• Training models for assay specificity analysis based on the cross-reactivity results with non-target bacteria.
• Optimizing molecular diagnostic protocols based on the reported 1-hour process time and 12 CFU/ml sensitivity.

Strengths

• Method achieves a reported limit of detection as low as 12 CFU/ml.
• Specificity testing confirmed no cross-reactivity with four listed non-target bacterial species.
• The entire detection process is described as being completed within 1 hour.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is a 1.1 MB DOCX file, suggesting a small-scale, document-based report rather than a structured data table.

Provenance

Source

figshare

Collection Method

Experimental results from the development of a recombinase polymerase amplification-based chromogenic assay.

Time Range

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Freshness

Last updated 2026-04-10 05:58:48; freshness should be verified.

Geography

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