Impact of Glutaraldehyde Cross-Linking on Allergen Polymer Structure and Allergenicity

A research study investigating how varying glutaraldehyde-to-protein ratios affect the structure and allergenicity of polymerized pollen extracts from Phleum pratense and Betula verrucosa. The dataset includes results from structural characterization methods like SDS-PAGE, NMR, TEM, and MS, as well as immunological assays for IgE reactivity and immunogenicity. The work was authored by Sandra Sivill and last updated on March 18, 2026.

Use Cases

• Analyzing the relationship between cross-linking stoichiometry and polymer size/density based on NMR and TEM data.
• Evaluating the reduction in allergenicity based on IgE reactivity measured by ELISA and mast cell activation tests.
• Comparing the immunogenic profiles of polymerized versus native allergens based on serum-specific IgG and lymphocyte response data.

Strengths

• Data is derived from a systematic study using five distinct cross-linking conditions.
• Structural characterization employs multiple complementary techniques: SDS-PAGE, NMR, TEM, and MS.
• Allergenicity is evaluated through three different assays: ELISA, Western blotting, and MAT.
• The dataset is openly available under a CC-BY-4.0 license.

Limitations

• The dataset is a 42.2 KB DOCX file, indicating a very limited data scope, likely a summary document.
• Column-level documentation and sample data are unavailable, requiring manual inspection after download.
• Row count is unknown, which may limit suitability assessment for quantitative analysis.

Provenance

Source

figshare

Collection Method

Experimental study involving polymerization of pollen extracts and subsequent structural/immunological analysis.

Freshness

Last updated 2026-03-18 07:47:25