Impact of Glutaraldehyde Cross-Linking on Allergen Polymer Structure and Allergenicity

A 40.4 KB document by Sandra Sivill, last updated March 18, 2026, presents research on polymerized allergen extracts. The study systematically investigates how varying glutaraldehyde-to-protein ratios affect the structural and allergenic properties of pollen extracts from Phleum pratense and Betula verrucosa. It includes results from structural characterization and allergenicity assays.

Use Cases

• Modeling the relationship between cross-linking stoichiometry and polymer size/density based on NMR and TEM data mentioned in the description.
• Training models to predict allergenicity reduction based on polymer structural features like stability and peptide profiles.
• Analyzing immunogenicity profiles of polymerized extracts for vaccine development based on serum-specific IgG and lymphocyte response data.

Strengths

• Data is derived from a systematic study using five different glutaraldehyde-to-protein ratios.
• Structural characterization includes multiple methods: SDS-PAGE, NMR, TEM, and mass spectrometry.
• Allergenicity was evaluated through three distinct assays: ELISA, Western blotting, and mast cell activation test.
• Published under a permissive CC-BY-4.0 license.

Limitations

• The dataset is a 40.4 KB DOCX file, indicating a very limited data scope.
• Row count and column-level documentation are unknown, requiring manual inspection after download.
• The data is specific to two pollen species (Phleum pratense and Betula verrucosa), limiting generalizability.

Provenance

Source

Sandra Sivill via figshare.

Collection Method

Experimental study polymerizing pollen extracts and characterizing the resulting polymers.

Freshness

Last updated 2026-03-18 07:47:24.