Impact of Glutaraldehyde Cross-Linking on Allergen Polymer Structure and Allergenicity

A 75.0 KB Excel dataset from a 2026 study by Sandra Sivill on figshare. It contains results from polymerizing pollen extracts from Phleum pratense and Betula verrucosa using five different glutaraldehyde-to-protein ratios. The data includes structural characterizations and allergenicity assessments of the resulting polymers.

Use Cases

• Modeling the relationship between cross-linking ratio and polymer size based on SDS-PAGE and TEM data
• Correlating polymer density from NMR with reduced allergenicity measured by ELISA and MAT
• Comparing the immunogenicity of polymerized versus native extracts based on serum-specific IgG and lymphocyte response data

Strengths

• Data is derived from a systematic study using five distinct cross-linking conditions.
• Polymer characterization includes multiple methods: SDS-PAGE, NMR, TEM, and mass spectrometry.
• Allergenicity was evaluated through three distinct assays: ELISA, Western blotting, and mast cell activation test.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small at 75.0 KB, indicating limited scope.

Provenance

Source

Sandra Sivill via figshare

Collection Method

Experimental study polymerizing pollen extracts and characterizing the resulting polymers.

Freshness

Last updated 2026-03-18 07:47:35; freshness should be verified.