Impact of Glutaraldehyde Cross-Linking on Allergen Polymer Structure and Allergenicity

Data from a study investigating five different glutaraldehyde-to-protein ratios used to polymerize pollen extracts from Phleum pratense and Betula verrucosa. The dataset includes structural characterization results from SDS-PAGE, NMR, TEM, and MS, alongside allergenicity and immunogenicity assays. It was authored by Sandra Sivill and last updated on 2026-03-18.

Use Cases

• Modeling the relationship between cross-linking ratio and polymer size/density based on NMR and TEM data.
• Training classifiers to predict allergenicity reduction based on structural polymer features.
• Analyzing correlations between polymer stability (from NMR) and reduced IgE reactivity (from ELISA and MAT).

Strengths

• Includes data from five distinct cross-linking stoichiometry conditions.
• Combines multiple characterization methods: SDS-PAGE, NMR, TEM, MS, ELISA, Western blot, and MAT.
• Dataset is openly available under a CC-BY-4.0 license.

Limitations

• Row count and column-level documentation are absent; field semantics must be inferred after download.
• The dataset is small at 118.6 KB, indicating limited scope.

Provenance

Source

Sandra Sivill via figshare.

Collection Method

Experimental data from polymerizing and characterizing allergen extracts.

Freshness

Last updated 2026-03-18 07:47:33; freshness should be verified.