Impact of Glutaraldehyde Cross-Linking on Allergen Polymers, 5 Stoichiometry Ratios

Five distinct glutaraldehyde-to-protein ratios were used to polymerize pollen extracts from Phleum pratense and Betula verrucosa. Sandra Sivill published this dataset on figshare in March 2026, detailing the resulting polymers' structural and allergenic properties. The 135.6 KB Excel file contains data on polymer size, morphology, yield, and reduced IgE reactivity.

Use Cases

• Modeling the relationship between cross-linking stoichiometry and polymer size/morphology based on NMR and TEM data
• Predicting allergenicity reduction in polymerized extracts based on IgE reactivity measurements from ELISA and MAT
• Comparing immunogenic responses elicited by high-density polymers versus native extracts using serum-specific IgG data

Strengths

• Data is derived from a systematic study using five different glutaraldehyde-to-protein ratios
• Polymer characterization employs multiple techniques: SDS-PAGE, NMR, TEM, and mass spectrometry
• Allergenicity is evaluated through three distinct assays: ELISA, Western blotting, and mast cell activation test

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download
• The dataset is small at 135.6 KB, indicating a limited experimental scope

Provenance

Source

Sandra Sivill via figshare

Collection Method

Experimental study polymerizing pollen extracts and characterizing the resulting polymers.

Freshness

Last updated 2026-03-18 07:47:33; freshness should be verified