Impact of Cross-Linking Stoichiometry on Polymerized Allergen Structure and Allergenicity

Experimental data from a study investigating how varying glutaraldehyde-to-protein ratios affect the structural and allergenic properties of polymerized pollen extracts. The dataset includes results from structural characterization techniques and immunological assays for extracts from Phleum pratense and Betula verrucosa. It was authored by Sandra Sivill and last updated on March 18, 2026.

Use Cases

• Modeling the relationship between cross-linking ratio and polymer size/density based on NMR and TEM data
• Analyzing the correlation between polymer structural properties and reduced IgE reactivity based on ELISA and MAT results
• Comparing peptide profiles of native and polymerized allergens using mass spectrometry data
• Evaluating the immunogenicity of optimized polymer formulations based on serum-specific IgG and lymphocyte response data

Strengths

• Data is derived from a systematic study using five distinct cross-linking conditions
• Structural characterization employs multiple techniques: SDS-PAGE, NMR, TEM, and mass spectrometry
• Allergenicity is evaluated through three complementary assays: ELISA, Western blotting, and mast cell activation test

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download
• The dataset is small at 70.7 KB, indicating limited scope

Provenance

Source

Sandra Sivill via figshare

Collection Method

Experimental study polymerizing pollen extracts and characterizing the resulting polymers.

Freshness

Last updated 2026-03-18 07:47:33; freshness should be verified