Impact of Glutaraldehyde Cross-Linking on Allergen Polymer Structure and Allergenicity

Five different glutaraldehyde-to-protein ratios were used to polymerize pollen extracts from Phleum pratense and Betula verrucosa. The dataset contains results from structural characterization (SDS-PAGE, NMR, TEM, MS) and immunological assays (IgE reactivity, IgG response, mast cell activation) for the resulting polymers. The data was authored by Sandra Sivill and last updated on 2026-03-18.

Use Cases

• Modeling the relationship between cross-linking ratio and polymer size/density based on NMR and TEM data.
• Predicting allergenicity reduction based on IgE reactivity measurements from ELISA and mast cell activation tests.
• Analyzing the correlation between polymer structural properties and immunogenic IgG responses in mice.

Strengths

• Data is derived from a systematic study using five distinct cross-linking conditions.
• Multiple analytical techniques (SDS-PAGE, NMR, TEM, MS, ELISA, Western blot, MAT) were used for characterization.
• License is CC-BY-4.0, permitting open sharing and adaptation.

Limitations

• Dataset is small at 48.8 KB, indicating limited scope.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

Sandra Sivill via figshare.

Collection Method

Experimental data from polymerizing pollen extracts and analyzing their structural and immunological properties.

Freshness

Last updated 2026-03-18 07:47:28; freshness should be verified.