Impact of Glutaraldehyde Cross-Linking on Allergen Polymer Structure and Allergenicity

Experimental data from a study investigating how five different glutaraldehyde-to-protein ratios affect the structure and allergenicity of polymerized pollen extracts from Phleum pratense and Betula verrucosa. The dataset includes results from structural characterization techniques like SDS-PAGE, NMR, TEM, and MS, and immunological assays such as ELISA, Western blotting, and mast cell activation tests. It was authored by Sandra Sivill and last updated on March 18, 2026.

Use Cases

• Modeling the relationship between cross-linking ratio and polymer size/density based on NMR and TEM data.
• Training models to predict allergenicity reduction (IgE reactivity) from polymer structural features.
• Analyzing correlations between peptide profile retention (from MS data) and immunogenicity (IgG response).

Strengths

• Data is derived from a systematic study using five distinct cross-linking conditions.
• Structural characterization employs multiple complementary techniques (SDS-PAGE, NMR, TEM, MS).
• Allergenicity is evaluated through three different immunological assays (ELISA, Western blot, MAT).
• Dataset is openly available under a CC-BY-4.0 license.

Limitations

• The dataset is small (138.7 KB), indicating limited scope and likely a summary of experimental results.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for statistical modeling.

Provenance

Source

Sandra Sivill via figshare.

Collection Method

Laboratory experiments polymerizing pollen extracts and characterizing the resulting polymers.

Freshness

Last updated 2026-03-18 07:47:27; freshness should be verified.