Impact of Glutaraldehyde Cross-Linking on Allergen Polymer Structure and Allergenicity

Sandra Sivill's study, last updated March 2026, provides data on the structural and immunological properties of polymerized allergen extracts. The dataset likely contains results from experiments using five different glutaraldehyde-to-protein ratios on pollen extracts from Phleum pratense and Betula verrucosa. It includes measurements of polymer size, morphology, yield, and assessments of allergenicity and immunogenicity.

Use Cases

• Modeling the relationship between cross-linking ratio and polymer size based on SDS-PAGE and TEM data
• Analyzing correlations between polymer density (from NMR/TEM) and reduced IgE reactivity (from ELISA/MAT)
• Comparing the immunogenic profiles of polymerized versus native extracts based on serum-specific IgG and lymphocyte response data

Strengths

• Data is derived from a systematic study using five distinct cross-linking conditions.
• Multiple analytical techniques (SDS-PAGE, NMR, TEM, MS, ELISA, Western blot, MAT) were used for characterization.
• Dataset is openly available under a CC-BY-4.0 license.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small at 133.5 KB, indicating limited scope.

Provenance

Source

Sandra Sivill, via figshare.

Collection Method

Experimental data from polymerizing pollen extracts and characterizing the resulting polymers.

Freshness

Last updated 2026-03-18 07:47:26; freshness should be verified.